luciferase reporter assay, does condition of debris, bubble, cell stimulation make change on result?

Matilda Ok @Matilda-Ok

07 February 2020 0 4K Report

I just started learning dual luciferase assay now.( Firefly-Renilla signal)

using HEK 293 cell, and give stimulation by PMA/lono.

the main point is I wonder what if I changes the few conditions, what experiment result make differences? and how?

please let me know what can be changed and the reasons for that.... or how can I get those informations ..

main question is below

4 conditions changed

1. harvest cell include cell debris without centrifuge.

2. less or much amounts of stimulation concentration

3. if I use different concentrations of cell, what is the main differences on data result.

4. do bubbles can distract analysis ?

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