How much protein yield is expected from 50 murine islets (assuming average 2 K cells per islet)?

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

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How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

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01 March 2021 2,027 2 View

Why do my MCFs not form monolayer?

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27 February 2021 3,904 2 View

What's the concentration recommend for recombinant protein use in western blotting?

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24 February 2021 8,514 3 View

Are there lab markers/pens resistant to Roti-Histol?

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24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

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22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

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21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

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Santosh H. S. Kumar

Are you looking for protein modification or just looking for the protein expression in the western blot???

for expression studies if it is ECL u r doing u can get fairly good amount of protein in detectable concentration.

if it is for looking protein modification, it depends on the Ab u r using,

Jay Patankar

Hi Santosh, Im looking forward for western blotting but would eventually would also like to fractionate the homogenates. thanks

Aaron M Herndon

I want to know, too. I am harvesting feline islets and I am able to get roughly 600ng of RNA from 50 islets. I have the protein but have not quantified it yet. Still sitting in the -80 :)