Hi researchers
i plan to do a study of genetic polymorphism associating with primaquine therapy in our population. I was wondering how much of venous blood should i take from patients for DNA extraction before storage in consideration of keeping a few as a back up. And how does the extraction go? Extract the whole of volume and transfer them into few aliquots. I plan to do 3x at a time from 3 aliquots and find the SD for one patient and i should have remaining 3 aliquots as back up for future use, just in case.
Thanks
Liza