Norliza Mat Ariffin

7 Questions 15 Answers 0 Followers

Questions related from Norliza Mat Ariffin

Phenotype assessment on variants of drug metabolizing enzyme system?

Any established formula to calculate metabolite to parent compound ratio to indicate the enzyme activity of CYP P450. I have two sampling time to indicate the elimination rate constant of parent...

01 January 2018 8,200 2 View

RFLP and Sequencing

Hi How does the preferences between these two methods play a role in genetic polymophism test? Can we choose one over the other? What are the circumstances that we use both? Thanks

06 June 2017 2,591 2 View

Should we optimise annealing temperature for ready PCR mastermix?

Let's say if we purchased ready master mix from certain manufacturer and the instruction says that Ta is based on Tm of primer (my F primer Tm is 59 and R primer Tm is 62), then how we can set the...

02 February 2017 2,688 3 View

Smearing band on a gel after PCR

My sample of DNA after PCR could be degraded probably due to PCR process. I used 3 step PCR cycles, Tm of forward primer was 59C and Tm of reverse primer was 62C. i chose 60C in this case. I am...

02 February 2017 8,510 5 View

Using old stuff for biology work

hi Currently there is stuff here left by previous PhD student like molecular biology reagent (Sigma Brand) which i think i want to send it for autoclaving. Is it ok? It was opened Jan 2013. Thanks

02 February 2016 4,268 2 View

How much of venous blood to be taken for PCR analysis?

Hi researchers i plan to do a study of genetic polymorphism associating with primaquine therapy in our population. I was wondering how much of venous blood should i take from patients for DNA...

12 December 2014 7,303 5 View

Preparation of DNA PCR product before gel electrophoresis

I have prepared DNA PCR product and the purity of each sample were within the ratio of 1.8-2, between 680ng/ul to 1200ng/ul using KOD FX Neo. I used precast gel 1.2 % agarose gel (Invitrogen) and...

01 January 1970 528 12 View