RFLP and Sequencing

More Norliza Mat Ariffin's questions See All

Phenotype assessment on variants of drug metabolizing enzyme system?

Any established formula to calculate metabolite to parent compound ratio to indicate the enzyme activity of CYP P450. I have two sampling time to indicate the elimination rate constant of parent...

31 December 2017 8,133 2 View

Should we optimise annealing temperature for ready PCR mastermix?

Let's say if we purchased ready master mix from certain manufacturer and the instruction says that Ta is based on Tm of primer (my F primer Tm is 59 and R primer Tm is 62), then how we can set the...

01 February 2017 2,600 3 View

Smearing band on a gel after PCR

My sample of DNA after PCR could be degraded probably due to PCR process. I used 3 step PCR cycles, Tm of forward primer was 59C and Tm of reverse primer was 62C. i chose 60C in this case. I am...

01 February 2017 8,448 5 View

Using old stuff for biology work

hi Currently there is stuff here left by previous PhD student like molecular biology reagent (Sigma Brand) which i think i want to send it for autoclaving. Is it ok? It was opened Jan 2013. Thanks

01 February 2016 4,208 2 View

How much of venous blood to be taken for PCR analysis?

Hi researchers i plan to do a study of genetic polymorphism associating with primaquine therapy in our population. I was wondering how much of venous blood should i take from patients for DNA...

11 December 2014 7,210 5 View

Preparation of DNA PCR product before gel electrophoresis

I have prepared DNA PCR product and the purity of each sample were within the ratio of 1.8-2, between 680ng/ul to 1200ng/ul using KOD FX Neo. I used precast gel 1.2 % agarose gel (Invitrogen) and...

01 January 1970 458 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Fawaz Al-Shaheri

Hi Norliza,

DNA sequencing is preferable, it is more accurate for mutation detection, than RFLP especially if the SNP have not specific Restriction site. However, it is costly than RFLP. RFLP only reveal SNP. SO., the other types of mutations are only detectable by squencing

Taghi Ghassemi-Khademi

Sequencing is better than RFLP