Using old stuff for biology work

More Norliza Mat Ariffin's questions See All

Phenotype assessment on variants of drug metabolizing enzyme system?

Any established formula to calculate metabolite to parent compound ratio to indicate the enzyme activity of CYP P450. I have two sampling time to indicate the elimination rate constant of parent...

31 December 2017 8,234 2 View

RFLP and Sequencing

Hi How does the preferences between these two methods play a role in genetic polymophism test? Can we choose one over the other? What are the circumstances that we use both? Thanks

05 June 2017 2,652 2 View

Should we optimise annealing temperature for ready PCR mastermix?

Let's say if we purchased ready master mix from certain manufacturer and the instruction says that Ta is based on Tm of primer (my F primer Tm is 59 and R primer Tm is 62), then how we can set the...

01 February 2017 2,720 3 View

Smearing band on a gel after PCR

My sample of DNA after PCR could be degraded probably due to PCR process. I used 3 step PCR cycles, Tm of forward primer was 59C and Tm of reverse primer was 62C. i chose 60C in this case. I am...

01 February 2017 8,548 5 View

How much of venous blood to be taken for PCR analysis?

Hi researchers i plan to do a study of genetic polymorphism associating with primaquine therapy in our population. I was wondering how much of venous blood should i take from patients for DNA...

11 December 2014 7,367 5 View

Preparation of DNA PCR product before gel electrophoresis

I have prepared DNA PCR product and the purity of each sample were within the ratio of 1.8-2, between 680ng/ul to 1200ng/ul using KOD FX Neo. I used precast gel 1.2 % agarose gel (Invitrogen) and...

01 January 1970 559 12 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View