Using old stuff for biology work

More Norliza Mat Ariffin's questions See All

Phenotype assessment on variants of drug metabolizing enzyme system?

Any established formula to calculate metabolite to parent compound ratio to indicate the enzyme activity of CYP P450. I have two sampling time to indicate the elimination rate constant of parent...

31 December 2017 8,133 2 View

RFLP and Sequencing

Hi How does the preferences between these two methods play a role in genetic polymophism test? Can we choose one over the other? What are the circumstances that we use both? Thanks

05 June 2017 2,506 2 View

Should we optimise annealing temperature for ready PCR mastermix?

Let's say if we purchased ready master mix from certain manufacturer and the instruction says that Ta is based on Tm of primer (my F primer Tm is 59 and R primer Tm is 62), then how we can set the...

01 February 2017 2,600 3 View

Smearing band on a gel after PCR

My sample of DNA after PCR could be degraded probably due to PCR process. I used 3 step PCR cycles, Tm of forward primer was 59C and Tm of reverse primer was 62C. i chose 60C in this case. I am...

01 February 2017 8,448 5 View

How much of venous blood to be taken for PCR analysis?

Hi researchers i plan to do a study of genetic polymorphism associating with primaquine therapy in our population. I was wondering how much of venous blood should i take from patients for DNA...

11 December 2014 7,210 5 View

Preparation of DNA PCR product before gel electrophoresis

I have prepared DNA PCR product and the purity of each sample were within the ratio of 1.8-2, between 680ng/ul to 1200ng/ul using KOD FX Neo. I used precast gel 1.2 % agarose gel (Invitrogen) and...

01 January 1970 458 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View