Hi guys, I'm doing a oil palm marker study. This is my first time using BSA. I have a few questions.
1) Can anyone suggest the concentration and amount of BSA that is commonly used in PCR?
2) How to prepare BSA for PCR as my BSA is in pellet form?
Thanks =)
Hi...
It depends on your PCR master mix and DNA quality. Normally we use 0.2 ug/lL BSA (50 µg/ml), i.e., If you prepare a master mix for on plate 96 well (0.06*96=5.76 µL).
For the second part of your Q, lock at your tube they write how much is the BSA concentration (50 µg/ml) or more then you.
good lock.
Hi,
I have 100mg of BSA pellet and I use premix master mix from Nexpro. Do you mean you take 0.2µg from BSA stock? And can I know how do you prepare 50µg/ml of stock BSA (eg. add H2O or NaCl etc.)?
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