Lim Wan Wan

7 Questions 9 Answers 0 Followers

Questions related from Lim Wan Wan

How to extract SNP flanking sequence from own unpublished plant reference genome?

I have the SNP information (VCF) and would like to extract its flanking sequence. How do I extract it from my reference genome? Do I need to write scripts and programs for it? Thank you.

21 May 2021 4,348 4 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

04 June 2020 4,951 3 View

How many SSR are enough in per chromosome for genetic diversity study? How to choose suitable SSR?

Hi, I have 200++ SSR markers across genome, but I don't have enough resources to test all the SSRs on my samples. And I want to select SSRs that are distributed uniformly in each chromosome. How...

21 June 2018 7,490 7 View

Why 2,4-dichlorophenoxyacetic does not kill plant cells?

Hi, I'm new to plant tissue culture. As I know, synthetic auxin like 2,4-dichlorophenoxy acetic acid (2,4-D) and 2,4,5-trichlorophenoxy acetic acid (2,4,5-T) are used for callus induction and are...

19 November 2016 4,996 9 View

Why can't I find short sequence containing SNP in original gene sequence?

Hi guys, I'm trying to find a short sequence which has SNP in the original gene sequence. But I can't find any match at all in the gene sequence, not even a small fragment. SNP...

12 May 2016 979 3 View

How much of BSA is needed in plant marker PCR?

Hi guys, I'm doing a oil palm marker study. This is my first time using BSA. I have a few questions. 1) Can anyone suggest the concentration and amount of BSA that is commonly used in PCR? 2) How...

21 April 2016 6,993 2 View

Is fatty acid composition the only factor affecting oil palm's oil quality?

I'm trying to find if there is any genetic factor affecting the oil quality.

02 June 2015 2,868 3 View