M. Eugenio Vazquez

7 Questions 145 Answers 0 Followers

Questions related from M. Eugenio Vazquez

Why do we observe a low value in anisotropy in the first point of a titration with DNA?

We are measuring the anisotropy of a small oligocationic molecule after each addition of a DNA solution. The titration profile fits rather well to a 1:1 binding mode (as we expected), but the...

09 September 2017 8,738 9 View

What would be a good buffer model of the mitochondrial matrix (i.e., pH, [H2O2], specific ions, etc)?

Hey, I'm interested in testing a ROS-sensitive probe in vitro. Is there a standard buffer that people uses to reproduce the conditions (pH, oxidative medium, ionic strenght, etc.) of the...

07 July 2016 4,133 4 View

Any references out there about extensive mutation of surface residues by Arg?

I've found some papers describing improved solubility and thermodynamic stabilization by mutating a few surface residues to Arg. However, these studies typically describe mutants with very few...

04 April 2015 5,939 2 View

Can someone recommend a good experimental protocol for EM of Biotin/Streptavidin-Gold NP?

We have a molecule tagged with biotin, and then we want to use streptavidin-gold Nanoparticle conjugates to localize it in electron microscopy. Had anybody tried it and has a good experimental...

01 January 2014 8,608 6 View

What would be the best strategy to synthesize an oligopeptide containing aldehyde side-chains?

I would like to make short oligopeptides with aldehyde side chains. My first thought is oxidation of oligo-homoserine peptides (Fmoc-L-Hse(Trt)-OH is commercially available, and therefore very...

10 October 2013 9,348 3 View

Conditions for a selective Buchwald-Hartwig amination?

Let's say I have 2-bromophenyl trifluoromethanesulfonate and I want to perform a Buchwald-Hartwig amination. Would it be possible to selectively modify the OTf with one amine, and THEN the bromide...

12 December 2012 2,222 5 View

Anyone can suggest a good review about peptide sequences that direct molecules to specific organelles?

I know about NLS, and some of the mitochondrial, but is there any good (and recent) review covering those sequences and the proposed mechanisms for their action? Thanks

10 October 2012 3,702 4 View