How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

What is your opinion about defining web development as a scientific research area?

In last years, web development added new layers of opportunities for research, as more metrics like loading time, payload and resources requests are defined. In that case, the development has new...

01 November 2020 8,090 7 View

Does anyone knows a data simulator for mobile phones?

I am interested in a software that generates the network events data sent by a mobile phone to the surroundings antennae. At least a good tutorial about what data the mobile phone sends to the...

15 March 2019 4,915 5 View

High standard erros in Amos (for moderation effects). Why?

Hello, I have built a SEM model in Amos, containing interaction effects between latent construct and a dummy variable. I think I have made everything by the book: (1) I have centered the latent...

10 July 2018 6,291 1 View

Are there any methods to deal with modelling the self-selection bias for count statistics?

Suppose that we want to count the individuals in a population, but but we can count only a sub-population ( only those individuals that respond to a survey, or are for example subscribers to a...

03 July 2017 6,433 3 View

Determination of the lattice parameter of the GaN / AlN layers of a superlattice from TEM images

Hello!I was faced with the problem of determining the deformation from TEM images in layers of Gan / AlN superlattices (Fig. 1). I understand that to calculate the deformations I need to determine...

01 July 2017 1,487 8 View

Factors influencing the use of innovative teaching approaches?

Does anyone know of any recent literature review on the variables or factors that may influence teachers' enactment of innovative (i.e., socio-constructivist, reformed based, student-centered) vs....

27 June 2017 8,706 9 View

How much does it cost to sequence a cyanobacterial (bacterial) genome?

23 March 2017 5,169 3 View

Can CloudSim be used to simulate a cluster of HTTP server with a load balancer?

I played a little with CloudSim and looked over the examples provided in their repository. From the examples it is not clear to me if CloudSim can simulate HTTP servers that receive data about...

29 June 2016 3,275 3 View

What really are multiple tests in regression?

Suppose I have one data set with 4 outcome measures. I also have 6 predictors and then I can create 9 interaction terms. I run a model with all main effects (6) and 9 interaction terms for each of...

31 May 2015 588 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View