I have cloned a gene containing own promoter. If I insert CMV promoter upstream of this gene, this gene have two promoter. How much will increase the expression of the gene?
Probably a ton. At the mRNA level you could easily detect 50-100 fold more expression by adding the CMV promoter. This effect might be less dramatic if the endogenous promoter is quite strong. However, in general, the CMV promoter has higher transcriptional output than endogenous promoters across multiple (but not all) cell types.
If this is to assess in vitro, then the expression will be directly proportional to the available molecular quantity of the un oxidized amino acid, regardless of the numerical value of the promoters.