19 Questions 7 Answers 0 Followers
Questions related from Mohammad Shayestehpour
We want to evaluate cytotoxicity effect of a bacterial peptide on HeLa cells. Should we dilute the bacterial peptide in medium with serum (FBS) or medium without serum? When use medium with serum...
06 July 2021 2,832 3 View
How to calculate cytotoxicity concentration 50(CC50) by probit regression in Spss software?
09 June 2020 5,236 1 View
HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?
11 November 2015 8,910 3 View
I have a problem with gateway technology and LR reaction.I Perform LR reaction with various vector concentrations but the reaction is not carryout. I did check LR enzyme and this enzyme worked...
30 October 2015 9,329 2 View
I have cloned a gene containing own promoter. If I insert CMV promoter upstream of this gene, this gene have two promoter. How much will increase the expression of the gene?
13 August 2015 7,323 3 View
I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...
23 July 2015 10,023 2 View
I cloned an insert to a vector. Then I cloned another sequence (insert 2) to previous cloned vector.After transformation and performing colony PCR with designed primers for vector backbone, I...
16 July 2015 1,318 5 View
can i digest and ligate the PCR product to vector by ECORI and BSP119I? I using fast digest Thermo Scientific enzymes.
29 June 2015 2,094 5 View
I have a vector with 5308bp length.I digested this vector by NotI and ECORI enzymes. I must be observe two bands 1346bp and 3962bp but I observed 1346bp and 2500bp. What I observed band at 2500bp...
25 April 2015 8,978 3 View
I inserted a 3131 bp fragment to a vector (3661bp) by ECORI and BSP119I enzymes. I performed the colony PCR and saved the positive colonies after cultivation. When I extracted cloned plasmid from...
15 April 2015 5,848 8 View
I inserted a gen to a vector. I must insert a synthetic sequence at this gen by blunt end cloning. At the first stage of cloning (insertion a gen to a vector) is removed negative selection marker...
08 April 2015 5,756 2 View
I insert a gen to a vector. I must insert a synthetic sequence at this gen by blunt end cloning. At the first stage of cloning (insertion a gen to a vector) is removed negative selection marker...
08 April 2015 1,901 3 View
i cut a plasmid by HPAI enzyme (creation blunt end) and dephosphorylate simultaneously. after run on gel, 2 band observed. i nead to linearized plasmid for cloning. How can I decipher between...
07 April 2015 9,604 6 View
NK cells or interferon alpha?
28 December 2014 7,454 18 View
for a research i need to Adenovirus type 5. Does anyone have the adenovirus type 5 stock?
02 December 2014 1,595 1 View
I have purchased a vector (PENTR11) from Invitrogen. The concentration of this vector is 0.5 ug/ul. How much vector (ng) should be added to competent Ecoli for propagation and maintenance of the...
29 November 2014 8,966 2 View
Can a ligation reaction can be stored in the freezer and used again? Does anyone have a frozen ligation product?
23 November 2014 585 11 View
I used from High Fidelity PCREnzyme Mix (thermo scientific:product number k0191) for PCR performing.My PCR product is 3131bp from HEK-293 cell line DNA.On the first day, I performed PCR test but...
10 September 2014 5,686 47 View
I used from High Fidelity PCREnzyme Mix (thermo scientific:product number k0191) for PCR performing.My PCR product is 3131bp from HEK-293 cell line DNA.On the first day, I performed a PCR test but...
09 September 2014 2,670 13 View
