Are there any researchers who have compared dry and wet grinding results under laboratory conditions with Life Cycle Assessment?

Dear Researchers, I would like to compare the environmental load of dry and wet grinding under laboratory conditions (later with the scale-up method also for operating conditions). I have already...

31 October 2020 6,703 11 View

Can you provide RT-PCR raw data to train our machine learning algorithm?

Dear colleagues working with RT-PCR devices, We are a team of data scientists, biologists, web developers working on an open-source machine-learning algorithm to contribute suppressing the COVID19...

06 May 2020 1,789 3 View

CHO-K1 and HEK-293 most efficient transfection method?

Hello. I try to transiently transfect CHO-K1 and HEK-293 for protein expression. To estimate ( microscopically ) the efficiency of transfection I use pAcGFP1-N1. I have tried to transfect with...

30 April 2020 9,295 2 View

Any projects/institutions for an internship in Art Therapy?

26 November 2019 6,201 4 View

How to calculate Odds ratio, but with different baselines risks of individuals ?

I hope someone can help me, Im trying to analyse a sample in which the dependent variable is death and the independent belonging to a category (Disease Type A, B,C etc.). All individuals were...

23 October 2019 3,180 3 View

How to combine the scale-up model with the LCA model in building industry?

The results of the concrete experiments can be used to build up a model to determine the power consumption of an equipment and to establish the scale-up method. The Life Cycle Assessment for...

12 June 2019 1,442 3 View

Concerning the Eratigena atrica group - which stage is shown and which filaments are produced by the different spinnerets?

Image 1 and 2 show the juvenile spider from a dorsal and ventral view. What is the stage? Is it sub-sub-adult? length 7mm. Image 3 shows the the 3x2 different spinnerets. What are the functions...

14 March 2018 3,516 1 View

Does somebody have experiences in targeting one type of mRNA from one species in a complex microbial mixture?

Dear all, currently, I am investigating mouse stool samples on the presence of a certain mRNA from a certain species with 2-step RT-qPCR. The problem with fecal samples is that there are numerous...

07 February 2017 1,938 1 View

Can anyone please recommend relevant literature (articles) on breast carcinomas of rats, where is describes about PCNA, mast cells, imunity, mitosis ?

Thank you

18 August 2015 8,078 2 View

Can lucifer yellow be used multiple times on the same Caco2 culture?

We don't have a 96well TEER measurement capability, but I wanted to make sure that the tight junctions are good prior to experimentation. Can lucifer yellow be used prior to the experiment to...

16 February 2015 7,492 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View