Dear Viktoria,
have you tried to decalcify the eye before embedding the sample for cryosections? That would help as it dies in paraffin sections.
The following protocol was used
Fixation in parapharmaldehyde 4%\10%, fixation with formalin 4%\10% + fixation in sucrose 30%\10%
Or parapharmaldehyde 4%10%, fixation with formalin 4%10% + decalcification of sodium citrate and formic acid. But there is still the effect of "blinds" and the slices break
What is the size of the eye and how many days are you doing the decalcification? Might not be enough time for the size of the lens. Another question, is it just the centre of the lens that still has the dettachment effect?
The size of the lens is up to 0.5 cm, decalcification is 2 days, the solution changes every 24
The lens breaks not only in the center, but also on the periphery
It not such a small lens, and breaking on the periphery as well is clearly a sign that the decalcification solution is not penetrating the tissue. I would suggest that you keep in decalcification solution for 1 to 2 weeks changing the solution every 2-4 days. One other thing that could help the penetration of the solution in the eye is a small cut on the cornea. Of course, consider these suggestions only if they do not interfere with the aim of your experiments.
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