What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

What is the difference between SEM and TEM and between FTIR and EDX in soil and plant?

If I want to know the changes (for example in plants or soils) as affected by specific substance on nano substance; when I use SEM or TEM and why? As well, when I use FTIR or EDX and why also? Is...

28 January 2021 5,070 9 View

Rmse in lstm when forecasting time series?

iam working on long memory time series data and i use lstm neural networ to train data and i want to understand how rmse is calculated is it calculated in each iteration separately or in each...

23 December 2020 6,740 4 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

I saw sparkles in my samples?

i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why

16 November 2020 6,852 5 View

I am looking for efficient texture analysis software for MRI images analysis with free acess?

i am looking for efficient texture analysis software for MRI images analysis with free access?

09 October 2020 3,737 2 View

What is the alternative test that can be used instead of ANCOVA when the Homogeneity of regression slopes doesn't meet?

I have Pre test as a (covariate variable) and post test as a (dependent variable) and the treatment for the experimental group and the control group. and the assumption of Homogeneity of...

21 August 2020 7,196 3 View

What if find that the reviewer goes through your paper just to reject it?

What you should do if you found that one of the reviewers goes through your paper just to reject it and most of his comments are irrational and not constructive. Finally, he rejected the paper...

20 April 2020 5,336 51 View

Giemsa stain preparation tips, for chromosomes' staining, any advises?

Can any one advise me about the best protocol for Giemsa stain preparation? it gives me faint blue color not the ideal purple color, that makes the investigation difficult and the color is not...

16 March 2020 6,656 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View