In my opinion, you should use it only one time.

Because heating the solution can change concentration of the different component of the fixative liquid.

Regards,

VM

Thanks a lot!

We use a portion of Bouins once or twice a week for at least half a year without bad outcome as pretreatment for trichromes.

We prepare the Bouin's fixative whenever we need it, we never reuse it.

I agree to Gudrun, I use Bouin solution during 6 months and don't observe any difference in the results 

Thanks to Gudrun and Rita and Andres too! My question arises from an attempt to waste as less as possible Bouin's solution . I'll surely follow your advices! I'll try to re-use it at least for half a year, I mean till the onset of  troubles! Regards, Marika

Yes, Bouin's can be re-used.  I agree with Gudrun and Rita. 

If you were staining with the Masson's Trichrome everyday or multiple runs a day, then I would change it after a month.  If you don't stain with Masson Trichrome often, six months sounds like a reasonable time frame.

In histological studies, we usually use it one time .

Regards Gh.  

Thanks Louisa and Ghadire.

The fixative is used only once. Greetings from Belgrade, Serbia

In histological and histochemical studies we normally use it one time.

Thanks Senka.

And thanks Opinder...

In histological studies we use it one time.

Thnaks Ghadire