We have been washing treated culture dishes with hipoclorite and extran and iradiating to use them again. How many times can we do that without detaching or stressing the cells? Do you have any experience with this?
Here are related discussions that may be helpful:
https://www.researchgate.net/post/Can_tissue_culture_flasks_be_reused
https://www.researchgate.net/post/Can_I_reuse_flasks_and_petri_dishes
https://www.researchgate.net/post/Can_media_from_one_cell_culture_flask_be_reused
Hello Ana Sayuri Yamagata
Please refer to the article below for more information.
Article Microwave irradiation of plastic tissue culture vessels for reuse
Highlight:
A simple protocol has been developed for recycling plastic tissue culture vessels. The killing properties of microwaves were used to decontaminate plastic tissue culture vessels for reuse. Nine bacterial cultures, four gram-negative and five gram-positive genera, including two Bacillus species, were used to artificially contaminate tissue culture vessels. The microwaves produced by a "home-type" microwave oven (2.45 gHz) were able to decontaminate the vessels with a 3-min exposure. The same exposure time was also used to completely inactivate the following three test viruses: polio type 1, parainfluenza type 1 (Sendai), and bacteriophage T4. The recycling procedure did not reduce the attachment and proliferation of the following cell types: primary chicken and turkey embryo, HEp-2, Vero, BGMK, and MK-2.
Please Note:
At present, we do not reuse our flasks more than four times. After the third cycle through the microwave oven, the plastics begin to show a yellowing effect, indicating that the plastic may be breaking down.
Best.
- Badges
- Science topic
- Similar topics
- Biological Science
- Physiology
- Physiological Processes
- Stress