Hi everyone!
I would like to start researching the response of human mast cells to yeast. Which commercial human mast cell line do you think is the best? I have read that LAD-2 is difficult to passaging and ect. What do you think about HMC1.2 and LUVA?
Hello Marta Rogalska
The two known human mast cell lines, HMC-1 and LAD2, are derived from patients with mast cell leukemias. The HMC-1 cell line represents very immature transformed cells and they contain no chymase, very little tryptase, and do not express functional IgE receptors.
LAD2 cells are functional mast cells but have a widely variable doubling time, an unstable phenotype in culture, and can be difficult to freeze and recover.
On the other hand, LUVA cells respond to IgE receptor cross-linkage and resemble mature human mast cells functionally and morphologically. They do not exhibit autophosphorylation of the c-kit receptor and respond to recombinant human c-kit ligand, the stem cell factor, with accelerated proliferation. However, neither the stem cell factor nor any other exogenous growth factor is necessary to maintain the survival or proliferation of LUVA cells. LUVA cells are the first mast cell line derived from a patient with no clinical mast cell disorder and no mutation in the c-kit receptor. They should be valuable for studies of human mast cell function.
I recommend the use of LUVA cell line for your study.
You may want to refer to the articles attached below for more information.
Article Preclinical human models and emerging therapeutics for advan...
Article Mast cell lines HMC-1 and LAD2 in comparison with mature hum...
Article Characterization of a novel human mast cell line that respon...
Best.
Thank you so much for your advice and references! It's very helpful.
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