Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

How to extract big peripheral membrane proteins?

I have been trying for a long time to see by Western Blot a 450 kDa-peripheral membrane protein from the Golgi apparatus with no success. I know that the electrophoresis and the membrane transfer...

28 September 2020 9,664 3 View

Maiden vs married name?

When I claimed authorship of papers written under my maiden name, they have now appeared under my married name, and I am worried this will cause confusion. How do I fix this issue?

12 August 2020 6,936 5 View

Rat DRG survival issue - how can I debug the protocol?

Hello everyone, I have been trying to establish primary rat DRG neuron culture for a couple of months already. I am using the protocol I found somewhere during the Google search, apparently...

14 June 2020 343 1 View

How to clean analytical size exclusion column?

Hello, We are using Superdex75 increase 3.2/30 GL size exclusion column in our lab and recently column pressure is to high to work on recommended flow rate 0.1 mL/min. I tried to clean the column...

19 May 2020 8,549 4 View

Unexpected problems running Photoquad software: How to fix the issue when it doesn't run anymore?

Hi, I've been using Photoquad software to analyze photoquadrats but suddenly the program doesn't run anymore and I am not able to solve the problem. I uninstalled both the MATLAB Compiler...

21 April 2020 4,707 1 View

Did any of you use polyethylene glycol to break down antibodies in Western Blot?

Hi, I've got a problem with my Western Blot. I have a low weight protein to detect but I suspect that my primary and secondary antibodies are clumping into a large ball at a higher molar mass than...

09 March 2020 2,856 3 View

Can anyone tell me the results of European Xenome research?

I know that the European Union has funded XENOME, an integrated project within the Sixth European Framework Programme (Life Sciences, Genomics and Biotechnology for Health), lasting 5 years...

18 November 2019 6,532 2 View

Hi all, is it possible to use XBridge BEH Amide Column to separate acetoin and 2,3-butanediol via HPLC?

Most of the methods that I have found recommend Aminex HPX-87H but I would rather avoind buying new column. Thanks, Marta

13 November 2019 6,221 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View