Dear Researchers,
I would like to start working with Caco-2. I have seen in articles that different media (e.g. MEM or DMEM with various suplementation) are used to culture Caco-2. Which is the best (and no so expensive) medium for the Caco-2 cell line?
MEM with L-glutamine and extra + 1% NEAA +10% FBS + 1% Pen-Strep
DMEM (but which?) and extra + 1%NEAA + 10% FBS 1% Pen-Strep
Should I add sodium pyruvate or sodium bicarbonate?
Thanks for your time
Marta
Hello Marta Rogalska
MEM is much better than DMEM and it should be supplemented with 10-20% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1.5 g/L sodium bicarbonate and 1% Penicillin-Streptomycin (pen/strep) antibiotic solution.
You need not add sodium bicarbonate, if it is already present in the media. There is no need to supplement the media with sodium pyruvate.
Best.
Hello,
I would use the medium that the supplier you bought the Caco-2 cell line from recommend, e.g. ATCC or ECACC, see file and link
https://www.culturecollections.org.uk/nop/product/caco-2-2
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