How many passages do embryonic cells continue surviving under G418 selection?
Is it defined what mechanism enables ESC from skipping such stress? is it a molecular program including ubiquitation changes?
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
steps scopes
07 April 2024 1,171 1 View
Field notes: Where do you write in mixed research in any chapter? In the procedures chapter or the results chapter? "In doctoral thesis"
31 March 2024 5,472 3 View
How do we study the expressions that contribute to the spread of extremism in society and how do we work to confront them through expressions that encourage peaceful coexistence?
25 January 2024 6,912 1 View
Can anyone of you please share the right conceptual model for this variables? I have two Independent variable, One dependent variable, one moderator, and one mediator. So now I could not find...
24 December 2023 398 0 View
I need your help as soon as possible, thank you
10 November 2023 7,998 0 View
If we take a metal which is loaded elastically (near the yield point but still elastic) and heat it to a sufficient temperature, such that its yield strength drops, some of the elastic strain will...
24 September 2023 2,885 2 View
thank you
24 September 2023 1,348 0 View
Is a lactic acid bacteria strain sourced from plant sources is more suitable for fermentation of plant proteins compared with a dairy sourced strain?
18 June 2023 2,805 1 View
I am working on a project to use bacterial fermentation of proteins from plant-based resources. I was wondering if anyone comment on which lactic acid bacteria i.e. Lactobacillus helveticus or L....
18 June 2023 690 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View