What is the impact of Washback in teaching a language or any other subject?

The importance of washback in teaching and learning the language through assessment

15 November 2020 3,559 4 View

How can we use 'assemblage theory' by multi-disciplines for designing a better children environment?

A research group is conducting a study across several cultures to investigate insights about issues in the environment of school-age children (6-12). The purpose is to explore practitioners'...

05 August 2020 1,967 5 View

Can you help in the two-minute survey about nightlife atmospheres in historical places?

19 July 2020 9,584 15 View

Load deflection curve in abaqus?

Ihahve modeled RC beam in abaqus, but the load decrease extremely after the maximum is reached, what could be the problem?

03 May 2020 2,566 8 View

What is the difference between connector type axial and friction in abaqus to define a bond between concrete and steel?

what is the best way to simulate the bond between steel and concrete if i will use connector, what is the difference between connector type axial and friction how i can define shear stress...

25 April 2020 5,880 3 View

Can I use anhydrous ammonium phosphate in oil and grease extraction?

hi, can I use anhydrous ammonium phosphate instead of anhydrous sodium sulfate in oil and grease extraction?

23 March 2020 9,987 3 View

Is test of significance(p value) necessary in some researchs??

hi, i'm writing a research about decolorization of waste water by fungi. the results obtained by % decolorization. Is it necessary to include test of significance??

29 December 2018 7,535 3 View

What's the typical active area for anode and cathode for EIS test?

EIS, Active areas of Anode, Active area of cathode, SOFC

16 November 2018 3,137 4 View

Can you help me ,i need resent researches on orthogonality in Banach Space?

I begin anew research on this topic

09 September 2018 9,062 5 View

How to count cells on micro carriers using trypan blue ?

I have cells attached on PLGA-MC in spinner flask and I want to get daily 1 ml sample of cells-MC in order to draw growth curve "proliferation" for my cells. the problem that I cant get the...

30 May 2018 9,135 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View