Abeer Heskol

5 Questions 4 Answers 0 Followers

Questions related from Abeer Heskol

Does BSA in washing buffer after fixation affect sonication?

Hello all, I add BSA in PBS that I use for cell collection after fixing cells for ChIP prep to avoid cells adhering on the flask/tube walls. I was wondering if BSA concentration might affect the...

08 December 2020 4,599 3 View

Can you re-sonicate an under sonicated chromatin?

Hello all, I want to ask if you can proceed with under-sonicated chromatin by thawing on ice and sonicating extra cycles, will affect/compromise the chromatin quality laters with IP? I am doing...

08 December 2020 6,672 3 View

Does anyone know why I am getting a shorter product than expected for RNA libraries?

I'm running reverse transcription experiments to amplify single cell RNA. I do lysis for the cells, RT and then pcr amplification.The library products I'm getting from cell samples are shorter...

14 January 2016 6,012 5 View

How many passages do embryonic cells continue under G418 selection?

How many passages do embryonic cells continue surviving under G418 selection?

22 October 2015 5,921 1 View

Is it possible that the B-cells after fate selection in Germinal Centers (GC) will proliferate outside the GC?

After maturation and differentiation (into memory or plasma cells) in the germinal centers inside secondary lymphatic organs, Do B-cells expand and proliferate outside or inside the germinal...

10 March 2015 1,960 2 View