How to dissolve alpha melanocyte hormone (alpha-MSH)for long storage?

I am going to use alpha-MSH to stimulate B16F10 melanin production. I bought 1mg alpha-MSh from sigma. According to sigma, the protein dissolve in water. Can I dissolve the alpha-MSH in pbs, or...

06 December 2018 9,851 2 View

How to Identify Polymers of Packaging?

Dear all researchers, I have problem on identifying type of polymer used in different type of food packaging. I need to screen many food packaging in marketplace that use polymer that I want. In...

25 February 2018 4,232 3 View

How to change the solvent used in preparing standard stock solution?

Hi, can I know how to change solvent used in my standard stock solution? For your information, I initially prepared the standard (styrene) in methanol and I need to change it to hexane. Can I...

05 February 2018 2,935 3 View

DPPH Assay for enzymatic antioxidant?

Assalamualaikum and Good Day. I am currently working with enzymatic antioxidants such as SOD, CAT, APX etc in rice plants exposed to different water level (water stress treatment). I am a bit...

11 September 2017 3,456 3 View

I need to detect same compound using HPLC in different sample. Should I construct different calibration curve for each and every sample I studied?

I need to detect styrene monomer from different food sample

03 June 2017 3,662 10 View

How can I test the statisitcal significance of mean frequencies?

This is the situation: I observed 12 classes (4 elementary, 4 intermediate and 4 advanced) without any intervention or treatment. Each class was taught by a different teacher. So there were 12...

29 May 2017 1,228 4 View

Is Tafel plot suitable to determine corrosion rates of organic coatings?

I'm doing research about anticorrosive composite coatings by using different filler compositions (1%-5%). Currently I'm using EIS to study the anticorrosive behavior of my coatings. My question...

16 March 2017 4,781 7 View

Which package in R to implement the feature selection that suitable for regression?

15 January 2017 5,834 2 View

What are the suitable solvent extraction for liquid-liquid extraction ketamine?

liquid-liquid extraction of ketamine for chromatographic analysis

03 April 2016 9,248 1 View

Is there strong evidence in increasing estrogen from soy protein?

03 March 2016 3,091 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View