How many days (maximum) can CD4+ cells from human propagate in cell culture? Is there any any protocol to make them survive for longer periods?

02 February 2014 1 7K Report

I want to culture CD4+ cells for longer period.

Just add IL-2 and normal RPMI with 10% FCS, you should be able to get them going for a month with proper medium change. For antigen-specific T cells, check our publication: Chen PNAS p9363 2004 CD4. To go for longer term, deplete CD8 and CD25+ cells to start.

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