I want to know the genes in human which are in gene dense and gene desert region.
I want to culture CD4+ cells for longer period.
01 February 2014 7,473 1 View
I want to know whether splicing occurs inside speckles or outside.
11 December 2013 2,554 0 View
Alternative isoform producing genes characterization.
11 December 2013 4,805 2 View
I want to know how alternative splicing, speckles localisation and expression of gene is affected by its position in gene dense and gene desert region.
10 November 2013 6,452 0 View
Two variables show higher r value in log scale than linear. What is the imp of logarithmic R value in biology?
10 November 2013 3,764 4 View
I need to know the gene rich region on chromosome in human which localize in speckles for their expression and splicing compare to other genes which don't depend on speckles localization.
10 November 2013 6,913 0 View
Many people think that flu virus is the most clever. But how can we measure intelligence of virus and bacteria? Is that pathogenesis, escaping from immune system or no vaccine?
08 September 2013 6,725 5 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View