I want to know whether splicing occurs inside speckles or outside.
I want to culture CD4+ cells for longer period.
01 February 2014 7,473 1 View
Alternative isoform producing genes characterization.
11 December 2013 4,805 2 View
I want to know how alternative splicing, speckles localisation and expression of gene is affected by its position in gene dense and gene desert region.
10 November 2013 6,452 0 View
Two variables show higher r value in log scale than linear. What is the imp of logarithmic R value in biology?
10 November 2013 3,764 4 View
I need to know the gene rich region on chromosome in human which localize in speckles for their expression and splicing compare to other genes which don't depend on speckles localization.
10 November 2013 6,913 0 View
I want to know the genes in human which are in gene dense and gene desert region.
10 November 2013 3,834 0 View
Many people think that flu virus is the most clever. But how can we measure intelligence of virus and bacteria? Is that pathogenesis, escaping from immune system or no vaccine?
08 September 2013 6,725 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
