How many days (maximum) can CD4+ cells from human propagate in cell culture? Is there any any protocol to make them survive for longer periods?

I want to culture CD4+ cells for longer period.

01 February 2014 7,396 1 View

What are the characteristics of alternative isoform producing genes?

Alternative isoform producing genes characterization.

11 December 2013 4,725 2 View

Are the gene in gene-dense region more expressed compared to the gene in gene desert region?

I want to know how alternative splicing, speckles localisation and expression of gene is affected by its position in gene dense and gene desert region.

10 November 2013 6,369 0 View

What will be the conclusion if regression analysis is better logarithmically than linearly?

Two variables show higher r value in log scale than linear. What is the imp of logarithmic R value in biology?

10 November 2013 3,669 4 View

Where can I get the information about human genes which are enriched in speckles for either their expression or splicing?

I need to know the gene rich region on chromosome in human which localize in speckles for their expression and splicing compare to other genes which don't depend on speckles localization.

10 November 2013 6,837 0 View

Which region of human chromosomes are gene dense and gene desert and how it affect their localisation in speckles?

I want to know the genes in human which are in gene dense and gene desert region.

10 November 2013 3,725 0 View

Which virus can be considered as clever virus? Can we tell because it escapes from our immune system like HIV or are some viruses clever?

Many people think that flu virus is the most clever. But how can we measure intelligence of virus and bacteria? Is that pathogenesis, escaping from immune system or no vaccine?

08 September 2013 6,630 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View