How many days (maximum) can CD4+ cells from human propagate in cell culture? Is there any any protocol to make them survive for longer periods?

I want to culture CD4+ cells for longer period.

01 February 2014 7,396 1 View

Is there a link between speckles and splicing?

I want to know whether splicing occurs inside speckles or outside.

11 December 2013 2,477 0 View

What are the characteristics of alternative isoform producing genes?

Alternative isoform producing genes characterization.

11 December 2013 4,725 2 View

Are the gene in gene-dense region more expressed compared to the gene in gene desert region?

I want to know how alternative splicing, speckles localisation and expression of gene is affected by its position in gene dense and gene desert region.

10 November 2013 6,369 0 View

What will be the conclusion if regression analysis is better logarithmically than linearly?

Two variables show higher r value in log scale than linear. What is the imp of logarithmic R value in biology?

10 November 2013 3,669 4 View

Which region of human chromosomes are gene dense and gene desert and how it affect their localisation in speckles?

I want to know the genes in human which are in gene dense and gene desert region.

10 November 2013 3,725 0 View

Which virus can be considered as clever virus? Can we tell because it escapes from our immune system like HIV or are some viruses clever?

Many people think that flu virus is the most clever. But how can we measure intelligence of virus and bacteria? Is that pathogenesis, escaping from immune system or no vaccine?

08 September 2013 6,630 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View