I will use 6 well plates. I'm studying the migration of the macrophages? Is it possible cell be migrate after it cell already differentiate monocytes into macrophages?
A quick intuitive response to the question on whether PMA-differentiated THP-1 can migrate: No, as far as I know from imaging experiments. The cells become very large, spread out and don't look very active or motile. The differentiation primarily turns them to the gene expression profile needed to study macrophages, but that does not necessarily reconstitute the phenotype in vivo.
Questions about how to culture and differentiate THP-1 have been asked many times in this RG forum.
Briefly, the optimized dose is 5 ng/mL PMA. The exactly procedure can be found in this highly cited paper:
Article Optimized THP-1 differentiation is required for the detectio...
Previous Q&A: https://www.researchgate.net/post/I_have_got_new_cell_line_THP-1_can_anyone_tell_me_what_will_be_suitable_media_for_their_culture
Good luck!
http://sqm.org.mx/PDF/2017/memorias2017/01Memorias_BIOQ.pdf#page=14
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