Dear Experts!
Minimum how many elite accessions of mother plants have to be considered in developing tissue culture protocol. How does the number of accessions affect the efficiency of the tissue culture protocol of a species?
Thanks!
More than 1000 different accessions of shoot-tip cultures have been initiated in vitro, multiplied and maintained at reduced temperature conditions
A tissue culture protocol is a combination of various chemicals including growth regulators and defined set of steps/procedure with specific ex plants to get maximum number of plantlets. Within a species, the regeneration efficiency is variable and it dependent on the genotype of the accession/variety of the species. Except growth regulators, the remaining components of the tissue culture protocol remained by and large same for the same explant of a species. However, with different ex plants of the same species a different set of procedure is required. Thus, practical utilization and importance point of view, it is better to select the most promising accession of a species and standardized a protocol with most responsive ex plants. The developed protocol is required to be validated using another set of 5-10 potential but diverse accessions/variety of the species.
Dear M. Manokari it only depends of your particular conditions. From a side, you must to consider the availability of human resources. On the other side are your interests. The combination of both will give you the number of accesions you could manage. You must think from a practical point of view. Like for plant improvement, the higher the number of accesions evaluated, he higher the probability to succeed. However, the higher the number of accesions, also the higher the evaluators you would need. The species with you're working with is other key factor.
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