Cannot see ladder like patterns on gel (but sharp bands) after electrophoresis of LAMP amplicons. Reasons?

I am working on establishing a LAMP based protocol for detecting Yersinia enterocolitica- which is a food borne enteropathogn. I designed the three primer pairs (F3, B3, FIP, BIP, LF, LB) from...

01 January 1970 8,349 1 View