Tarun Tyagi

4 Questions 26 Answers 0 Followers

Questions related from Tarun Tyagi

How to denature pre-mRNA for RT-PCR ?

mRNA are easy to denature but denaturation of pre-mRNA requires additional steps. I have tried heating at 70C and 80C for 10 minutes prior to cDNA synthesis, but it hasn't worked yet, can...

10 October 2013 2,813 1 View

Can anybody tel me a method to cleave a long RNA fragment (pre mRNA) into fragments of 2-3 kb?

I want to amplify a region of a specific pre mRNA, but the standard method is not working.

10 October 2013 9,768 6 View

Is it possible to isolate WBCs or PBMCs from whole blood by Ficoll method without contamination of platelets, and if yes, how ?

I have tried isolating leukocytes from whole blood by Histopaque (sigma), but when I checked it in microscope, I found lots of platelets along with leukocytes, I tried washing, but it din't help much.

07 July 2013 2,018 1 View

can smone help me out in Intracellular calcium measurement by Fura 2AM method ?

03 March 2012 7,516 0 View