How to use the Embedded Region constrain on Abaqus CAE?

Hello fellow academics, For my Final Year Project, I am looking into different ways of modelling the interface between PVA and gelatin. I have successfully modeled the interface using the "tied"...

04 February 2021 8,698 1 View

Why does my Abaqus FEA simulation run forever?

Hello! I have recently been trying to model friction between two blocks of different materials in Abaqus FEA. I have started with a very simple model with two cubes of different materials next to...

17 October 2020 1,268 18 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

Any study on high spatial resolution water chemistry monitoring ?

We measured several water chemistry parameters (nitrate, DOC, EC, pH, Tre..) every 50m in the stream network of six headwater catchments. Has anybody done that before, and what geostatistical...

23 March 2020 7,355 12 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

When you have a nanoparticle stabilized by a polymer, is it more stable than a nanoparticle stabilized by a non-polymer ?

Any explanation or reference ? Thanks !

07 August 2018 4,705 6 View

CDC6 and Ori in bacteria

15 June 2017 4,895 1 View

Question about Crispr Cas system construction?

I want to constructed Crispr Cas system in archeae and I need to know which promoter and terminater I should use to express RNAguide. Indeed, if I understand well, promoter should be one for tRNA...

17 October 2016 1,260 3 View

How can I use a node displacement in a FRIC subroutine (in Abaqus) ?

Hello everyone, I am trying to model a friction behaviour in Abaqus. To do that, I use the FRIC subroutine. The model works fine in loading, but it changes when unloading, so I have to use a...

25 October 2015 3,984 3 View

Does anybody have some experience in the FRIC subroutine in Abaqus?

03 July 2015 8,136 9 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View