Which solvent is best for HPLC and MS analysis of Secondary metabolites?

I am doing fermentation based screening of novel secondary metabolites from Bacteria. Which solvent i should use for HPLC and MS analysis. Should I use a single or different set of solvents like,...

08 September 2018 3,794 4 View

Can we identify the Silent biosynthetic gene clusters using Transcriptomic (Micro-array) analysis. ? If yes how ?

I am working on Secondary metabolites, I have identified the gene clusters using the AntiSmash bioinformatics tools, now I am interested to find the Silent Biosynthetic gene cluster. can we...

08 September 2018 6,874 7 View

How can we check the interaction of a particular bacteria with same group of bacteria or with other group of bacteria. ?

Hello everyone; I am planing to study the interaction of a bacteria in a natural ecosystem. what would be the best strategy to find the interaction of a particular bacteria with same group as well...

08 September 2017 3,444 3 View

What is the molecular basis of Low pH resistance in A. niger.??

The A. niger is an an efficient producer of week acids. they produce acetic acid even when the pH drops to 2. what is the molecular basis of such resistance?? how the fungi survive under such...

02 March 2017 3,647 8 View

The E. coli generation time is 16-20 minutes. but if we look at the replication it takes about 38 minutes. how its possible. ?

I want to know about this phenomena. how it can be possible?

01 February 2017 1,151 1 View

How to combine 2 fragments of a gene using PCR?

Hello every one. I am studying the role of phosphorylation of TFII-I in neural precursor cells and Dopamine committed cells. I have done point mutation at 248 phospho site i.e change the codon...

10 November 2016 9,139 5 View

Does antibiotic resistance in bacteria gives any clue about antibiotic production?

It is understood that bacteria are resistant to its own antibiotics. I carried out the antibiotic resistance profile of the antibiotic producer bacterial isolates. The Isolates were resistant to...

09 October 2016 8,847 8 View

What might be the molecular and cellular basis of antibiotic resistance in antibiotic producing bacteria??

09 October 2016 1,926 6 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View