How long we can store bacterial sample in Trizol reagent at -80 'C? for RNA extraction. ? will this affect the RNA expression?

I am curious, that wither we can store the bacterial sample in Trizol reagent or not.? if yes for how long and will it affect the RNA expression or not. Thanks to all in advance :-)

31 December 2018 6,491 3 View

Which solvent is best for HPLC and MS analysis of Secondary metabolites?

I am doing fermentation based screening of novel secondary metabolites from Bacteria. Which solvent i should use for HPLC and MS analysis. Should I use a single or different set of solvents like,...

08 September 2018 3,794 4 View

Can we identify the Silent biosynthetic gene clusters using Transcriptomic (Micro-array) analysis. ? If yes how ?

I am working on Secondary metabolites, I have identified the gene clusters using the AntiSmash bioinformatics tools, now I am interested to find the Silent Biosynthetic gene cluster. can we...

08 September 2018 6,874 7 View

How can we check the interaction of a particular bacteria with same group of bacteria or with other group of bacteria. ?

Hello everyone; I am planing to study the interaction of a bacteria in a natural ecosystem. what would be the best strategy to find the interaction of a particular bacteria with same group as well...

08 September 2017 3,444 3 View

The E. coli generation time is 16-20 minutes. but if we look at the replication it takes about 38 minutes. how its possible. ?

I want to know about this phenomena. how it can be possible?

01 February 2017 1,151 1 View

How to combine 2 fragments of a gene using PCR?

Hello every one. I am studying the role of phosphorylation of TFII-I in neural precursor cells and Dopamine committed cells. I have done point mutation at 248 phospho site i.e change the codon...

10 November 2016 9,139 5 View

Does antibiotic resistance in bacteria gives any clue about antibiotic production?

It is understood that bacteria are resistant to its own antibiotics. I carried out the antibiotic resistance profile of the antibiotic producer bacterial isolates. The Isolates were resistant to...

09 October 2016 8,847 8 View

What might be the molecular and cellular basis of antibiotic resistance in antibiotic producing bacteria??

09 October 2016 1,926 6 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View