Muhammad Hayat

9 Questions 12 Answers 0 Followers

Questions related from Muhammad Hayat

How long we can store bacterial sample in Trizol reagent at -80 'C? for RNA extraction. ? will this affect the RNA expression?

I am curious, that wither we can store the bacterial sample in Trizol reagent or not.? if yes for how long and will it affect the RNA expression or not. Thanks to all in advance :-)

01 January 2019 6,546 3 View

Can we identify the Silent biosynthetic gene clusters using Transcriptomic (Micro-array) analysis. ? If yes how ?

I am working on Secondary metabolites, I have identified the gene clusters using the AntiSmash bioinformatics tools, now I am interested to find the Silent Biosynthetic gene cluster. can we...

09 September 2018 6,971 7 View

Which solvent is best for HPLC and MS analysis of Secondary metabolites?

I am doing fermentation based screening of novel secondary metabolites from Bacteria. Which solvent i should use for HPLC and MS analysis. Should I use a single or different set of solvents like,...

09 September 2018 3,855 4 View

How can we check the interaction of a particular bacteria with same group of bacteria or with other group of bacteria. ?

Hello everyone; I am planing to study the interaction of a bacteria in a natural ecosystem. what would be the best strategy to find the interaction of a particular bacteria with same group as well...

09 September 2017 3,513 3 View

What is the molecular basis of Low pH resistance in A. niger.??

The A. niger is an an efficient producer of week acids. they produce acetic acid even when the pH drops to 2. what is the molecular basis of such resistance?? how the fungi survive under such...

03 March 2017 3,700 8 View

The E. coli generation time is 16-20 minutes. but if we look at the replication it takes about 38 minutes. how its possible. ?

I want to know about this phenomena. how it can be possible?

02 February 2017 1,227 1 View

How to combine 2 fragments of a gene using PCR?

Hello every one. I am studying the role of phosphorylation of TFII-I in neural precursor cells and Dopamine committed cells. I have done point mutation at 248 phospho site i.e change the codon...

11 November 2016 9,243 5 View

What might be the molecular and cellular basis of antibiotic resistance in antibiotic producing bacteria??

My research work is on antibiotic production. My queries is that "why the producers bacteria are resistant to antibiotic it produces? what might be the possible molecular and cellular strategies...

10 October 2016 1,976 6 View

Does antibiotic resistance in bacteria gives any clue about antibiotic production?

It is understood that bacteria are resistant to its own antibiotics. I carried out the antibiotic resistance profile of the antibiotic producer bacterial isolates. The Isolates were resistant to...

10 October 2016 8,910 8 View