How long we can store bacterial sample in Trizol reagent at -80 'C? for RNA extraction. ? will this affect the RNA expression?

I am curious, that wither we can store the bacterial sample in Trizol reagent or not.? if yes for how long and will it affect the RNA expression or not. Thanks to all in advance :-)

31 December 2018 6,491 3 View

Which solvent is best for HPLC and MS analysis of Secondary metabolites?

I am doing fermentation based screening of novel secondary metabolites from Bacteria. Which solvent i should use for HPLC and MS analysis. Should I use a single or different set of solvents like,...

08 September 2018 3,794 4 View

Can we identify the Silent biosynthetic gene clusters using Transcriptomic (Micro-array) analysis. ? If yes how ?

I am working on Secondary metabolites, I have identified the gene clusters using the AntiSmash bioinformatics tools, now I am interested to find the Silent Biosynthetic gene cluster. can we...

08 September 2018 6,874 7 View

How can we check the interaction of a particular bacteria with same group of bacteria or with other group of bacteria. ?

Hello everyone; I am planing to study the interaction of a bacteria in a natural ecosystem. what would be the best strategy to find the interaction of a particular bacteria with same group as well...

08 September 2017 3,444 3 View

What is the molecular basis of Low pH resistance in A. niger.??

The A. niger is an an efficient producer of week acids. they produce acetic acid even when the pH drops to 2. what is the molecular basis of such resistance?? how the fungi survive under such...

02 March 2017 3,647 8 View

How to combine 2 fragments of a gene using PCR?

Hello every one. I am studying the role of phosphorylation of TFII-I in neural precursor cells and Dopamine committed cells. I have done point mutation at 248 phospho site i.e change the codon...

10 November 2016 9,139 5 View

Does antibiotic resistance in bacteria gives any clue about antibiotic production?

It is understood that bacteria are resistant to its own antibiotics. I carried out the antibiotic resistance profile of the antibiotic producer bacterial isolates. The Isolates were resistant to...

09 October 2016 8,847 8 View

What might be the molecular and cellular basis of antibiotic resistance in antibiotic producing bacteria??

09 October 2016 1,926 6 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View