Hello Junayed,

After few hours (4 or so) you can see already cells attached, but the exact time when most of them are attachedwill depend on many factors (cell line, fibronectin coating, number of cells, composition of the medium, etc...) In general, after adding the cells they must be undisturbed for at least 16h (overnight), although many protocols recommend 24 h.

Dear Capella, Thank you very much for your answer and explanation.

We use 0.2% gelatin to coat the surface and it takes about 2--4 hours for HUVECs to attach. It may take about the same time (or slightly less) for the cells to attach to fibronectin.

Thank you Dear Abha Sahni for your answer!

4-6 hours are enough.

You need to choose a quality medium kit.

Thank you Snow Nie for your answer!