Junayed Nayeem

7 Questions 9 Answers 0 Followers

Questions related from Junayed Nayeem

Hi all, can anyone suggest how long PFA storage of lung tissue sample would be the best before making the block for sectioning?

I preserve RAT lung tissue in PFA for histology such as H & E, EVG staining for assessing vascular remodelling. I want to get any suggestion regarding the duration of PFA storage of lungs. I...

18 November 2024 5,551 82 View

Hi all, Can anyone provide a reliable Immunohistochemistry protocol from frozen sections?

Any good paper reference will be highly appreciated! Many Thanks in Advance!

12 December 2023 2,475 2 View

Is the age of donors from whom any primary cells are derived affect the expression of target genes in that cells?

I have found different level expression of a gene at the same primary cells which derived from different-aged donors. Kind Thanks!

11 June 2020 5,414 0 View

Can any one suggest that, how long a signaling pathway remains active in a cell, after inducing by a ligand?

Different signaling pathways are active in the cell which are activated by different kinds of ligand. However, I want to know if there is any generalization on the time of an activated signaling...

10 June 2019 8,151 6 View

Can anyone suggest, the working concentration of PDGF ligand to induce the PDGFRs in PC-3 cells?

PDGF signaling is very important for cancer progression. Recently, it has been implicated in various cancers like prostate, breast and GIT cancer. I use 50ng/ml of RPMI medium to induce the...

10 March 2019 8,120 0 View

Can anyone suggest that what is the doubling time for PC-3 cells?

I am new in culturing the PC-3 cells. I have cultured in RPMI-1640 medium supplemented with 10% FBS. I have plated 200,000 cells per well of 6- well plate.

23 October 2018 4,277 1 View

How long time it's required for HUVEC cell to attached on the culture dish?

HUVEC cells are attached on Fibro-nectin coated culture dishes

08 August 2018 7,454 6 View