I stored the cells at -80 ᵒC in Mr Frosty and next day (due to involvement in some other tasks) I completely forgot to transfer the cells in liquid nitrogen. When I realized, it's already 3 days past. I was wondering if I could transfer the cells now in liquid nitrogen. All the time I used to store the cells in -80 ᵒC for overnight and the very next day transfer in liquid nitrogen. Will there be any harmful effect on the cells such as effect on cell viability, etc. because of this?
cells at -80 ᵒC in Mr Frosty and next day I completely forgot to transfeer?
Dear, first main point of the experiment, you should not forget any step. Some time inefficiency at most important step causes failing of full experiment and changes the results. So researcher should be careful all time.
For your question, results may be changes at long time and short time store producers.
How long the mammalian cells can be stored at -80 ᵒC (in Mr Frosty) before transferring the cells in liquid nitrogen?
Within a week, however even after months it can be done.
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