I stored the cells at -80 ᵒC in Mr Frosty and next day (due to involvement in some other tasks) I completely forgot to transfer the cells in liquid nitrogen. When I realized, it's already 3 days past. I was wondering if I could transfer the cells now in liquid nitrogen. All the time I used to store the cells in -80 ᵒC for overnight and the very next day transfer in liquid nitrogen. Will there be any harmful effect on the cells such as effect on cell viability, etc. because of this?

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