Tracking fluorescently labelled extracellular vesicles in tissue?

18 January 2022 1 4K Report

I have tissue treated with fluorescently labeled EVs. What is the best way to process the tissue for sectioning that can retain the fluorescence?

I have tried cryostat sectioning. But the tissue sections look dirty, not as good as paraffin-embedded sectioning. And paraffin-embedded sectioning needs to go through a harsh environment (high temperature, xylene) that diminishes/quenches the fluorescence.

Any suggestion here? Thanks

Sagar Rayamajhi

Jiri Trousil, do you have any recommended publication for PEG embedding protocol? Thanks!

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