Dear All
I would like to coat silicon tubings (Tygon) with 1% Pluronic F127 to prevent cell attachment inside the tubing. Can I use the coated tubings 1 day after I coated or should I use immediately after the coating? Thank you very much!
Best
Su
Dear All I would like to bond my PDMS chip to a glass but the size of my chip will be greater than a usual glass slide. Therefore, I need to bond it to a larger glass. Is that possible? Or can it...
11 October 2021 5,462 3 View
Dear All I would like to use a peristaltic pump that applies minimum shear stress on the roller part. It is also important to have a multi-channel pump since many parallel experiments at the same...
11 September 2021 9,260 3 View
Dear All I would like to use a peristaltic pump that exerts minimum occlusion in the rotating part of rollers, since cell flow will be inside the tubings. Therefore, is there a commercial...
06 September 2021 9,196 0 View
Dear All Can I place a peristaltic pump system with tubings and microfluidics chip completely into the incubator for 1-2 days long experiment? If yes, is there a specific brand for that...
05 September 2021 984 11 View
Dear All I am trying to plot shear stress values in 3D microchannels and I am using the formula spf.sr*spf.mu however I am getting different values from online microfluidic channel WSS...
19 July 2021 4,272 7 View
Dear All I am trying to simulate cells in a 2D laminar flow using two phase level-set method in COMSOL. I use p1 = 1000 kg/mˆ3 and dynamic viscosity u1 = 0.001 Pa.s for liquid and p2 = 1500...
16 July 2021 1,283 3 View
15 July 2021 7,229 1 View
Dear All I will be using a peristaltic pump with 4 or more tubings with flow rate range nearly 0.2 ml/min to 40 ml/min. Which peristaltic pump brand do you recommend? Thank you very much. Best...
15 July 2021 6,773 2 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View