Are RGD (Arg-Gly-Asp) peptides the right choice to block integrin-ligand interactions?

I am searching for a pan-inhibitor of the integrin signaling pathway. Would usage of the RGD (Arg-Gly-Asp) peptides be the right choice?

02 March 2014 553 4 View

Are Wnt proteins detectable by mass spectrometry?

Are Wnt proteins (hydrophobic due to palmitoylation) detectable by mass spectrometry?

09 October 2013 7,284 6 View

Which software is the best to quantify Western blot?

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08 September 2013 7,122 10 View

Are there some simple protein assays which could help me with protein identification before going into mass spectrometry?

Protein identification in cell culture supernatant

07 August 2013 9,406 14 View

What would be the best proliferation assay for epithelial organoids (3D culture)?

I am culturing epithelial organoids (isolated from small intestine) in matrigel. I would like to study proliferation. Does anyone know which proliferation assay is the most suitable for such culture?

07 August 2013 4,739 14 View

How to improve mouse tail injection?

I am a beginner in the field of bone marrow transplantation. I have a problem in that when performing mouse tail injection I usually do not see the vein. Does anyone know what is the best way to...

07 August 2013 6,860 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View