Does anyone know how long is IWP-2 (inhibitor of Wnt secretion) stable at 37°C?
I am searching for a pan-inhibitor of the integrin signaling pathway. Would usage of the RGD (Arg-Gly-Asp) peptides be the right choice?
02 March 2014 659 4 View
Are Wnt proteins (hydrophobic due to palmitoylation) detectable by mass spectrometry?
09 October 2013 7,374 6 View
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08 September 2013 7,239 10 View
Protein identification in cell culture supernatant
07 August 2013 9,512 14 View
I am culturing epithelial organoids (isolated from small intestine) in matrigel. I would like to study proliferation. Does anyone know which proliferation assay is the most suitable for such culture?
07 August 2013 4,847 14 View
I am a beginner in the field of bone marrow transplantation. I have a problem in that when performing mouse tail injection I usually do not see the vein. Does anyone know what is the best way to...
07 August 2013 6,963 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View