I am searching for a pan-inhibitor of the integrin signaling pathway. Would usage of the RGD (Arg-Gly-Asp) peptides be the right choice?
Only if RGD is the integrin ligand. If so, very high doses are usually required for inhibition (mM range). Many folks use RGDS peptide and RGES as peptide negative control.
Richard
Only if RGD is the integrin ligand. If so, very high doses are usually required for inhibition (mM range). Many folks use RGDS peptide and RGES as peptide negative control.
Richard
Agneiszka.
This depends on whether your objective is to inhibit ALL RGD-dependent interactions, specific integrin interactions, or specific ligand induced pathways.
Not all integrins use RGD as their sole target motif, and not all RGD's motifs are functional. (e.g there is one in BSA!)
Equally integrin-induced intracellular signalling cascades are not singular nor unidirectional.
I think you need to think carefully about the question you are asking and then devise an appropriate strategy. And please remember that when you block what you think is a unique target, it is highly unlikely it will function in isolation. Thus there WILL be off-target effects! How do you discriminate these from your objective?
Put simply, it isn't as simple as you would like to believe!
David L.
Hi. I need a list of natural RGD blockiing peptide.
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