I have been using 1 micromolar TTX to block spontaneous oscillations in the rd retina. It looks like TTX has its full effect only after 15 minutes of application. I was expecting much faster effect. Any explanation?
Petia Kupenova
In our experiments on frog eyecup preparations the effect of 3 muM tetrodotoxin on the ERG b- and d-waves developed in 10 minutes. Such slow development of the effects was found also for other toxins like picrotoxin (15 min). In the case of tetrodotoxin, an important reason may be the pH dependence of its effects. The time constant of its binding is increased and the efficacy of its action diminished in alkaline pH, because of the reduced availability of its cationic form, which is the active one.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line
More Shubhash Chandra Yadav's questions See All
Similar questions and discussions