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Questions related from Shubhash Chandra Yadav
I guess Quinine works the other way around, blocks Cx36 but not so much of Cx45.
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The resistance of the sharp electrode would be roughly 100MOhm. The goal is to study tracer coupling pattern of some retinal cells.
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I have been using 1 micromolar TTX to block spontaneous oscillations in the rd retina. It looks like TTX has its full effect only after 15 minutes of application. I was expecting much faster...
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If yes, when do you start to see the effect? Within hours of mounting or several days? Is it case specific? I have had nice stainings with several synaptic proteins/markers when using AF647 in...
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