Shubhash Chandra Yadav

6 Questions 5 Answers 0 Followers

Questions related from Shubhash Chandra Yadav

Is it possible to specifically block Cx45 gap junctions without affecting Cx36 gap junctions?

I guess Quinine works the other way around, blocks Cx36 but not so much of Cx45.

17 March 2022 9,148 2 View

Is it possible to inject dye/Neurobiotin (via sharp microelectrodes) using Multiclamp 700B patch clamp amplifier?

The resistance of the sharp electrode would be roughly 100MOhm. The goal is to study tracer coupling pattern of some retinal cells.

13 August 2021 1,765 1 View

How long does TTX take to block voltage gated sodium channels?

I have been using 1 micromolar TTX to block spontaneous oscillations in the rd retina. It looks like TTX has its full effect only after 15 minutes of application. I was expecting much faster...

06 August 2021 5,811 1 View

Does Vectashield really quench Alexa Fluor 647 fluorescence?

If yes, when do you start to see the effect? Within hours of mounting or several days? Is it case specific? I have had nice stainings with several synaptic proteins/markers when using AF647 in...

14 September 2020 2,743 1 View

How do you label horizontal cells in the mouse whole-mount retina prior to dye injections?

I use DAPI to label horizontal cells. However, they are rarely labelled. On the other hand, this strongly labels bipolar cells.

23 July 2020 2,582 5 View

Which cells (live) are predominantly labelled by DAPI in the mouse whole-mount retina? Are OFF bipolar cells better stained than ON bipolar cells?

In my DAPI stainings, I noticed that DAPI disparately labels retinal cells. Sometimes, ganglion cells are brightly labelled, other times they are faintly labelled. Usually, amacrine and bipolar...

02 December 2019 7,444 4 View