I transferred Saccharomyces cerevisiae yeast strain optimized to produce precursor of pathway with beta-carotene producing plasmid (CRTE, YB and I). There is constitutive promoter (TDH3) upstream of all 3 genes. For how many days should I wait to get orange color? Already the colonies are small but white.Or has some one also faced the same problem? I got some recommendation referring to change the temperature to 4 'C.
I am looking forward to your advice.
Hi there,
I think these should help:
http://aem.asm.org/content/73/13/4342.full
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922794/
Even by the 1st day there is some carotenoid production but most of the production takes place during the stationary phase. By day 3, the maximum production is reached.
Dear Par Naser, Can you let me know the place that you purchased the carotene producing yeast strain, because currently we need to purchase a similar kind of strain for a research purpose,
Thanks,
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