Par Naser

3 Questions 8 Answers 0 Followers

Questions related from Par Naser

Why my yeast strain, which is auxotrophic for Uracil, can grow on SD-Ura?

I have strain with ura3-52 mutation. It could growth on SD-Ura in my first attempt. So I thought there is contamination and I requested for new strain from our colleagues. I checked it again and...

29 July 2016 9,950 10 View

How long does it take yeast to produced beta-carotene?

I transferred Saccharomyces cerevisiae yeast strain optimized to produce precursor of pathway with beta-carotene producing plasmid (CRTE, YB and I). There is constitutive promoter (TDH3) upstream...

15 July 2016 5,276 4 View

How can IPTG be used to induce protein expression on yeast plate?

I am using inducible promoters to express ß-carotene in yeast S. cerevisiae cell. Yeast ß-carotene producing colonies are able to show orange color(light to dark). I transferred different promoter...

13 May 2016 5,493 4 View