Approximate time taken for lyophilization of 50 ml sample mixed in sterile water. Purpose of lyphilization is HPLC. We have Lyodel lyophilizer in our facility. Model: DPRG-1GH?
Thanks!
Hello Abirami,
we mostly lyophilize samples dissolved in acetonitrile/water (80/20). The volume can be up to 14 mL. We usually need 48h to freeze-dry this volume completely. At 50 mL I would rather estimate 2-3 days, so something between 48 and 72 hours. But you would have to try this in practice. But it can also depend on your sample. There are compounds that are strongly hygroscopic. They are not dry even after several days.
Regards
Markus
The majority of laboratory samples will lyophilize in 24 to 48 hours but there are certainly exceptions. The freeze drying process is dependent on a variety of factors that determine the amount of time the freeze drying process requires.
Sample type
The freezing temperature (eutectic point) of the sample and the presence of salts, sugars, or solvents will influence the length of time required to lyophilize successfully. A low freezing point sample or one that is susceptible to melt back, will require a longer lyophilization cycle.
Sample size
The more volume to lyophilize, the longer the freeze dry run.
Flask size
Spreading a sample out over a greater surface area allows more contact for heat input and shortens the distance in which the drying front has to move through the sample. As a general rule, freeze dry flasks should be filled to only one-third of their total volume.
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