Hello:
We want to develop a digital microfluidic platform with LAMP, but in this project planning I'm concerned with timing. Can someone who has worked with this platform help me with the time it takes to develop it and the description of the steps to get it?
We are very interested in getting into this topic but we have little experience, any support to raise the project correctly will be well received. Thank you very much to all of you.
I am not so much into LAMP specifically but I can tell you as a general rule for microfluidics: Longer than you expect...
Parameters I would need for an estimation:
1) How much routine do you have with microfludics, i.e. how many applications have you already developed
2) Do you need a proof of concept e.g. for an article in an academic journal or do you want to push it to industrial maturity.
3) Without giving away your creative idea, can you give some details on what elements you want to have on your chip beside LAMP?
Thanks for your answer, I will reach you to give you more details about the assay
If you already have a tentative design for the microfluidic platform, then you might be able to generate a final design within 3-4 iterations (at least that's what it takes in our lab).
Another major challenge would be LAMP primer design. Unlike PCR LAMP needs 6 primers, so it might take some time to make sure you are not getting false positive. So you might end up spending more time figuring out the LAMP reaction itself instead of device design.
Md. Nazibul Islam thank you so much. I´m just entering microfluidic`s world, I have experience in molecular diagnosis PCR and related techniques. This adaptation to how microfluidics work is the most challenging. So every piece of advice is taken into account
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