Yulaimy Batista Lozada

3 Questions 4 Answers 0 Followers

Questions related from Yulaimy Batista Lozada

How long does it take to develop a digital microfluidic platform with LAMP?

Hello: We want to develop a digital microfluidic platform with LAMP, but in this project planning I'm concerned with timing. Can someone who has worked with this platform help me with the time it...

04 October 2022 6,182 4 View

Have anyone tried SYBR green with GoTaq DNA Polymerase for a qRT-PCR?

Hi, everyone. I need to perform a real time reverse-transcription PCR in one step. What we have is SYBR green I PCR Master mix with GoTaq DNA Polymerase. So far, our attemps to perform the assay...

08 January 2021 6,660 3 View

I´m running a qPCR and I find 2 peaks in the melting curve, where there shouldn´t be any. Why is this happening?

The assay is to define if a genotype is present using 2 primers. The sample was extracted with Chelex from whole blood, and I know the person`s genotype, there shouldn´t be amplification and yet,...

11 December 2019 7,521 5 View